Enhancement of DNA-mediated gene transfer by high-Mr carrier DNA in synchronized CV-1 cells

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Abstract
Synchronized CV-1 cells were transfected with SV40 DNA-calcium phosphate co-precipitates. In the presence of carrier DNA, the transfection efficiency of SV40 DNA was decreased 5-fold in S-phase cells and was increased 4-fold in preparations of mitotically enriched cells, as compared with asynchronous controls. No difference was observed when: carrier DNA was omitted; cells had progressed through S-phase and into G2-phase; or the infectivity of cells to intact SV40 virus was tested. These results highlight the importance of cell-cycle-dependent factors on DNA-mediated gene transfer.