Catalytic Cysteine of Thymidylate Synthase Is Activated upon Substrate Binding,

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Abstract
The role of Ser 167 of Escherichia coli thymidylate synthase (TS) in catalysis has been characterized by kinetic and crystallographic studies. Position 167 variants including S167A, S167N, S167D, S167C, S167G, S167L, S167T, and S167V were generated by site-directed mutagenesis. Only S167A, S167G, S167T, and S167C complemented the growth of thymidine auxotrophs of E. coli in medium lacking thymidine. Steady-state kinetic analysis revealed that mutant enzymes exhibited kcat values 1.1−95-fold lower than that of the wild-type enzyme. Relative to wild-type TS, Km values of the mutant enzymes for 2‘-deoxyuridylate (dUMP) were 5−90 times higher, while Km values for 5,10-methylenetetrahydrofolate (CH2H4folate) were 1.5−16-fold higher. The rate of dehalogenation of 5-bromo-2‘-deoxyuridine 5‘-monophosphate (BrdUMP), a reaction catalyzed by TS that does not require CH2H4folate as cosubstrate, by mutant TSs was analyzed and showed that only S167A and S167G catalyzed the dehalogenation reaction and values of kcat/Km for the mutant enzymes were decreased by 10- and 3000-fold, respectively. Analysis of pre-steady-state kinetics of ternary complex formation revealed that the productive binding of CH2H4folate is weaker to mutant TSs than to the wild-type enzyme. Chemical transformation constants (kchem) for the mutant enzymes were lower by 1.1−6.0-fold relative to the wild-type enzyme. S167A, S167T, and S167C crystallized in the I213 space group and scattered X-rays to either 1.7 Å (S167A and S167T) or 2.6 Å (S167C). The high-resolution data sets were refined to a Rcrys of 19.9%. In the crystals some cysteine residues were derivatized with 2-mercaptoethanol to form S,S-(2-hydroxyethyl)thiocysteine. The pattern of derivatization indicates that in the absence of bound substrate the catalytic cysteine is not more reactive than other cysteines. It is proposed that the catalytic cysteine is activated by substrate binding by a proton-transfer mechanism in which the phosphate group of the nucleotide neutralizes the charge of Arg 126‘, facilitating the transfer of a proton from the catalytic cysteine to a His 207−Asp 205 diad via a system of ordered water molecules.

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