Nucleotide sequence analysis of DNA. 28. Arthrobacter luteus restriction endonuclease recognition sequence and its cleavage map of SV40 DNA
- 1 August 1976
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 15 (16) , 3612-3620
- https://doi.org/10.1021/bi00661a032
Abstract
The nucleotide sequence at the cleavage site of the restriction endonuclease isolated from A. luteus (Alu) was determined. The endonuclease cleaves at the center of a palindromic tetranucleotide sequence to give even-ended duplex DNA fragments phosphorylated at the 5''-end. The endonuclease cleaves SV40 form I DNA into 32 fragments. The order and sizes of these fragments were determined to provide an Alu cleavage map of the SV40 genome.Keywords
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