A Reagentless Amperometric Alcohol Biosensor Based on Carbon-Nanotube/Teflon Composite Electrodes

Abstract
We report on a multiwall carbon-nanotube (MWCNT)-based alcohol biosensor, based on the incorporation of alcohol-dehydrogenase/NAD+ within a three-dimensional MWCNT/Teflon matrix. The MWCNT component offers a marked decrease in the overvoltage for the oxidation of the liberated NADH along with minimal surface fouling effects, hence allowing convenient low-potential stable detection of the ethanol. The electrocatalytic detection is characterized, the biosensor composition and operation are optimized, and the analytical performance is illustrated.