Tricarboxylic acid cycle flux and enzyme activities in the isolated working rat heart

Abstract
Flux through the tricarboxylic acid cycle was calculated from O2 consumption in hearts perfused near the physiological work load. Activities of citrate synthase, 2-oxoglutarate dehydrogenase and succinate dehydrogenase were measured in the same hearts. Only the activities of 2-oxoglutarate dehydrogenase correlated with calculated fluxes through the cycle.