HYDRATION OF LYSOZYME IN AQUEOUS SOLUTION AS STUDIED BY SELF-DIFFUSION NMR MEASUREMENTS AND BY KERR-DIELECTRIC RELAXATION
- 1 September 1984
- journal article
- Published by EDP Sciences in Le Journal de Physique Colloques
- Vol. 45 (C7) , C7-255
- https://doi.org/10.1051/jphyscol:1984729
Abstract
An investigation of the hydration properties of HEW (hen-egg white) lysozyme in aqueous solutions (pH 4.7) has been carried out by measuring the self-diffusion coefficient of H2O using 1H NMR (spin echo technique) and by Kerr-dielectric relaxation using pulsed techniques. The 110 ± 15 water molecules inferred from Our NMR measurements correspond to the ordered water molecules interacting through hydrogen bonds with polar groups at the protein surface. From Kerr-dielectric relaxation measurements we compute the dimensions of the hydrodynamically equivalent ellipsoïd ie. 57 x 31 x 31 Å with a continous water monolayer 3.2 Å in thicknessKeywords
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