Electron microscope studies of deaminated and crosslinked collagen

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Abstract
SUMMARY: Glutaraldehyde‐crosslinked rat‐tail collagen, compared with native fibres, showed an increase in the size of the light bands when examined in the electron microscope using a negative‐staining technique. Collagen deaminated with nitrous acid gave similar patterns to native fibres and showed no change after treatment with glutaraldehyde. It was concluded that the increase in the size of the light bands is due to the formation of intermolecular crosslinks rather than charge neutralization of lysine amino groups.