Phosphate-Induced Protein Chromatography
- 21 December 1973
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 182 (4118) , 1268-1270
- https://doi.org/10.1126/science.182.4118.1268
Abstract
High phosphate concentration is shown to cause a large proportion of the proteins of a cell-free extract of Escherichia coli to bind to agarose columns to which L-valine is attached. With a decreasing concentration gradient of potassium phosphate, the proteins elute in relation to their solubility in concentrated ammonium sulfate. This column technique appears to provide a general tool for the purification of proteins.Keywords
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