Ecological Studies ofLegionellaSpecies

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Abstract
The occurrence and viable counts of Legionella pneumophila in acid-treated water samples of 62 cooling towers on the main island of Japan were determined by inoculating them onto plates of Wadowsky-Yee-Okuda (WYO) agar medium. WYO plate cultures of 39 (63%) of the samples yielded L. pneumophila with viable counts ranging from 10 to 104 colony-forming units per 100 ml. Of the L. pneumophila isolates, 157 were serologically identified as serogroup I, and the remaining 21 were agglutinated by serogroup 3 (2 strains) and serogroup 6 (19 strains) antisera. In each culture-positive water sample, the pH and the number of other bacteria were found not to be statistically significantly correlated with the viable counts of L. pneumophila. However, a higher rate of recovery of L. pneumophila was obtained with the water samples with a smaller number of ther bacteria. Practical use of commercially available antialgal or antimicrobial agents was found not be significantly effective for controlling the occurrence and growth of L. pneumophila in cooling tower water.