Enniatin Production byFusarium tricinctumand its Effect on Germinating Wheat Seeds

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Abstract
Fusarium tricinctum strains isolated from winter wheat with symptoms of crown and root rot, English ivy with leaf spot, apparently healthy red clover root, or pasture soil produced enniatins. Although enniatins are metabolites of at least five other species in the genus, this is the first report of their production by F. tricinctum. Analysis by mass spectrometry/mass spectrometry of extracts from 13 isolates showed that 10 of them were positive for enniatin with concentrations ranging from about 3 to 3.270 .mu.g/g of corn substrate. When enniatin (10-80 .mu.g/ml) was added to the solution in which wheat seeds were germinated, growth reduction of the developing seeds was directly related to the concenrated of enniatin, with root elongation being inhibited more than leaf development.