A Medium for Overcoming the in vitro Inhibition of Clostridium perfringens by Streptococcus faecalis var. zymogenes, and a Note on the in vivo Interaction of the Two Organisms

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Abstract
Summary. The presence of Streptococcus zymogenes in samples plated for the enumeration of Clostridium perfringens markedly reduces the numbers of clostridial colonies. This effect is not overcome by the use of media usually recommended for the selective isolation of Cl. perfringens, but an agar medium containing 100 μg of both D‐cycloserine and neomycin/ml proved satisfactory. Use of this medium for estimating numbers of Cl. perfringens in the gut of gnotobiotic quail has shown that inhibition by Strep. zymogene does not appear to occur in vivo.