Stimulation of yeast RNA polymerase II transcription by critical values of supercoiling

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Abstract
RNA chains of discrete length were obtained in vitro by yeast RNA polymerase II directed transcription of a supercoiled plasmid. On the basis of the amount and the MW of the RNA chains synthesized in the absence of reinitiation events, the number of actively transcribing RNA polymerase molecules was calculated. A stimulation of transcriptional activity was related to the torsional strength of negative supercoiling of the template. The DNA unwinding angle measured in the complexes formed with the enzyme in the presence of 3 ribonucleoside triphosphates equals 48.degree. .+-. 30.degree., marking a melting effect of 14 base pairs/bound enzyme molecule.

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