Quantitation of Flurazepam and Three Metabolites by Electron Capture Gas Liquid Chromatography

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Abstract
Flurazepam end three of its metabolic products (desalkyl, hydroxyethyl, end aldehyde metabolites) can be simultaneously quantitated without derivatization by gas chromatography with electron capture detection. After addition of a suitable internal standard, unknown biological samples and calibration standards are extracted at neutral pH Into benzene/isoamyl alcohol. The reconstituted extract is chromatographed at 275*C with a 10% OV-101 liquid phase, which allows resolution of all 5 compounds. In some cases a 1% OV-225 liquid phase is used for quantitation of hydroxyethylflurazepam. The method is sufficiently sensitive and reproducible for use in clinical and experimental pharmecokinetic studies.