Extension of the lifespan of cultured normal human diploid cells by vitamin E: a reevaluation.
Open Access
- 1 April 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (4) , 1640-1641
- https://doi.org/10.1073/pnas.74.4.1640
Abstract
It was previously reported that the lifespan of WI-38 human diploid [embryonic lung] fibroblasts in vitro was significantly increased by continuously growing the cell cultures in the presence of vitamin E (dl-.alpha.-tocopherol). In 19 subsequent subcultivation series these results were not reproducible. While vitamin E is incorporated into the cells and is able to act effectively as an antioxidant, apparently its intracellular antioxidant properties alone do not routinely result in an increase of cell lifespan. A synergism between vitamin E and some component(s) in the 1st of 2 lots of serum used in the original experiments seems the most likely explanation for the earlier findings.This publication has 3 references indexed in Scilit:
- PHOTOSENSITIZATION OF HUMAN DIPLOID CELL CULTURES BY INTRACELLULAR FLAVINS AND PROTECTION BY ANTIOXIDANTSPhotochemistry and Photobiology, 1976
- Extension of the Lifespan of Cultured Normal Human Diploid Cells by Vitamin EProceedings of the National Academy of Sciences, 1974
- Serum Albumin Supplemented Medium for Long Term Cultivation of Mammalian Fibroblast Strains.Experimental Biology and Medicine, 1964