Arabidopsis ABI5 Subfamily Members Have Distinct DNA-Binding and Transcriptional Activities
Open Access
- 1 October 2002
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 130 (2) , 688-697
- https://doi.org/10.1104/pp.003566
Abstract
A small family of novel basic leucine zipper proteins that includes abscisic acid (ABA)-INSENSITIVE 5 (ABI5) binds to the promoter region of the lea class gene Dc3. The factors, referred to as AtDPBFs (Arabidopsis Dc3 promoter-binding factors), were isolated from an immature seed cDNA library. AtDPBFs bind to the embryo specification and ABA-responsive elements in the Dc3 promoter and are unique in that they can interact with cis-elements that do not contain the ACGT core sequence required for the binding of most other plant basic leucine zipper proteins. Analysis of full-length cDNAs showed that at least five different Dc3 promoter-binding factors are present in Arabidopsis seeds; one of these, AtDPBF-1, is identical to ABI5. As expected, AtDPBF-1/ABI5 mRNA is inducible by exogenous ABA in seedlings. Despite the near identity in their basic domains, AtDPBFs are distinct in their DNA-binding, dimerization, and transcriptional activity.Keywords
This publication has 57 references indexed in Scilit:
- Salt Effects on Hydrophobic Interaction and Charge Screening in the Folding of a Negatively Charged Peptide to a Coiled Coil (Leucine Zipper)Biochemistry, 1998
- Regulation of gene expression programs during Arabidopsis seed development: roles of the ABI3 locus and of endogenous abscisic acid.Plant Cell, 1994
- bZIP proteins bind to a palindromic sequence without an ACGT core located in a seed‐specific element of the pea lectin promoterThe Plant Journal, 1994
- The transcriptional activator Opaque2 recognizes two different target sequences in the 22-kD-like alpha-prolamin genes.Plant Cell, 1994
- Plant bZIP Protein DNA Binding SpecificityJournal of Molecular Biology, 1993
- Hormonal and Environmental Regulation of the Carrot lea-Class Gene Dc3Plant Physiology, 1992
- Developmental and environmental induction of Lea and LeaA mRNAs and the postabscission program during embryo culture.Plant Cell, 1991
- Molecular analysis of a phylogenetically conserved carrot gene: Developmental and environmental regulationDevelopmental Genetics, 1989
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962