Binding and metabolism of the urinous odorant 5α-androstan-3-one in sheep olfactory mucosa

Publisher Website
Google Scholar
Abstract
The existence of specific receptors for the urinous smelling steroid 5α-androstan-3-one has been investigated in sheep olfactory mucosa using ligand-binding methods. [16,17-3H]5α-Androstan-3-one (52.5 Ci/mmol) was synthesized from the precursor 5α-androst-16-cn-3-one, which was prepared by two novel methods. A specific binding component, absent from control tissues was identified. The affinity constant was 9.6 ± 6.1 × 108 M-1 (mean ± SD) and competition experiments with other compounds indicate high specificity. Binding activity was localized in a high Mr tissue fraction and was distinct from 3-ketosteroid dehydrogenase and other enzymatic activity.

This publication has 10 references indexed in Scilit: