Cyclosporin-A- and Angiotensin-ll- Induced Vasoreactivity in Isolated Glomeruli and Cultured Mesangial Cells, 4 and 24 h after Renal Mass Reduction: Role of Vasodilatory Prostaglandins

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Abstract
Subtotal nephrectomy in the rat is followed by early hypertrophy and functional adaptation of the remnant glomeruli in which mesangial cells seem to play a leading part. Using a morphoquantitative approach, we assessed the vasoconstrictive response to 1 microM cyclosporin A (CsA) or 0.1 microM angiotensin II (AII) of isolated glomeruli and cultured mesangial cells obtained from normal rats and from rats after five-sixths nephrectomy. In normal rats, a significant reduction of glomerular size was observed after the administration of vasoactive agents (-14% after 30 min exposure to CsA or AII). After five-sixths nephrectomy the vasoconstrictive response, which was preserved at the 4th hour (-12% at the 30th minute), disappeared at the 24th hour (-1.5% at the 30th minute), but was maintained in animals pretreated with indomethacin (-10% at the 30th minute). Similar responses were observed with cultured mesangial cells at the first passage. Inhibition of prostaglandin synthesis abolishes the adaptative adjustments observed in partially nephrectomized rats during the first days after surgery. These findings suggest that renal vasodilatory prostaglandins may participate in the compensatory hemodynamic adjustments following subtotal nephrectomy.

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