Electrotransformation of Streptococcus pyogenes with plasmid and linear DNA

1 August 1991

journal article
Published by Oxford University Press (OUP) in FEMS Microbiology Letters

Vol. 82 (2) , 219-224
https://doi.org/10.1016/0378-1097(91)90336-9

Abstract

Electrotransformation was used to introduce both plasmid and linear DNA into Streptococcus pyogenes. The method was optimized using strain NZ131, for which transformation frequencies up to 10⁷ per μg of plasmid DNA were obtained. A linear fragment of DNA, containing the streptokinase gene (ska) in which an internal fragment had been replaced with an erythromycin resistance gene (erm), was transformed into strain NZ131 with a frequency of 10³ per μg DNA. The introduction of linear DNA into S. pyogenes by electrotransformation should be useful for future genetic analyses as well as targeted gene replacement.

Keywords

STREPTOCOCCUS PYOGENES
ELECTROTRANSFORMATION
GENE REPLACEMENT
STREPTOKINASE

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