The Demonstration of p32 in Bone by Radioautography
- 22 October 1948
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 108 (2808) , 445-448
- https://doi.org/10.1126/science.108.2808.445-a
Abstract
Rabbits were given intraven. 0.5 and 1.2 mc. of P32, respectively and killed next day. The bone was fixed in formalin containing 1-2% Pb acetate, washed, decalcified in 30% formic acid, embedded in paraffin, and sectioned. Radioautographs were made after mounting the sections on slides and pressing the dried sections against a high-speed, no-screen, x-ray film. Other sections were stained directly for histological study. It was determined that radioactive material is not transferred to non-radioactive areas during decal-cification. Since much radioactive material is removed during partial decalcification, it is necessary to use relatively large tracer doses. A preliminary profile is recommended.Keywords
This publication has 1 reference indexed in Scilit:
- An improved method for cutting undecalcified bone sections and its application to radio‐autographyThe Anatomical Record, 1947