The Detection of Monoclonal Macroglobulinemia with Antisera Specific for γM-Globulins
- 1 January 1967
- journal article
- research article
- Published by S. Karger AG in International Archives of Allergy and Immunology
- Vol. 31 (2) , 105-116
- https://doi.org/10.1159/000229859
Abstract
Rabbits were immunized against monoclonal macroglobulin (gammaM) of either type K or L. Some of these antisera, after absorption with gammaG, still could distinguish between normal gammaM (a mixture of type K and L) and the sera from patients with monoclonal macro-globulinemia. These antisera, when tested by double gel diffusion, formed spurs between the zones of precipitate of normal gammaM and the monoclonal gammaM globulins. If an anti KgammaM anti-serum was used, the spurs were formed with LgammaM globulin, and anti LgammaM globulin sera produced spurs with the KgammaM globulins. Absorption of the anti KgammaM antisera with gammaG and LgammaM resulted in antisera specific for KgammaM; similarly, absorption of anti LgammaM antisera with gammaG and KgammaM made them specific for LgammaM. The theory that antisera lacking antibodies against the light chains that determine the K or L specificity of the immunoglobulins still can be used for typing, is discussed.This publication has 1 reference indexed in Scilit:
- DIAGNOSIS OF MULTIPLE MYELOMA AND MACROGLOBULINEMIA BY THE OUCHTERLONY GEL DIFFUSION TECHNIQUE*Annals of the New York Academy of Sciences, 1962