Bioactive Surfaces via Immobilization of Self-Assembling Polymers onto Hydrophobic Materials
- 15 June 1999
- journal article
- research article
- Published by American Chemical Society (ACS) in Bioconjugate Chemistry
- Vol. 10 (4) , 678-686
- https://doi.org/10.1021/bc9800973
Abstract
Conjugation of proteins to copolymers from poly(acrylic acid) grafted onto PEO−PPO−PEO backbone (Pluronic-PAA) following adsorption of the conjugates onto hydrophobic surfaces is reported. Insulin-Pluronic-PAA conjugates show negligible internalization of insulin into human uterine smooth muscle cells as well as enhancement of mitogenic activity. Glucose-induced release of glycated albumin complexed with a Pluronic-PAA-concanavalin conjugate and adsorbed onto polystyrene nanospheres may provide a model for a glucose-responsive protein delivery system or a heterogeneous diagnostic device.Keywords
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