Development of a Homologous Radioimmunoassay for Secreted Hamster Prolactin

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Abstract
A specific and sensitive homologous radioimmunoassay was developed for secreted hamster prolactin. Hamster serum and pituitary extracts showed parallel dilution-response curves with hamster prolactin. The sensitivity of the assay ranged from 0.5 to 1.0 ng/ml, and the intra- and inter-assay coefficients of variation were 6 and 10%, respectively. Additionally it was demonstrated that the rat prolactin radioimmunoassay kit distributed by the National Institute of Arthritis, Metabolism and Digestive Diseases [USA] is an inadequate method for the measurement of hamster prolactin.