Direct Measurement of the pK Values of an Alkaline Bohr Group in Human Hemoglobin
- 1 April 1973
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 70 (4) , 1246-1249
- https://doi.org/10.1073/pnas.70.4.1246
Abstract
Proton nuclear magnetic resonance spectra of normal and des-(his 146beta) human hemoglobin in the aromatic resonance region have been compared at different pH values. From these measurements, a pK value for histidine 146beta of 7.1 in carbonmonoxyhemoglobin and 8.0 in deoxyhemoglobin has been found. These pK values confirm the proposed role of histidine 146beta in the alkaline Bohr effect.Keywords
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