Characteristics of Adrenergic Binding Sites Associated with Murine Lymphocytes Isolated from Spleen
- 1 January 1980
- journal article
- Published by Taylor & Francis in Journal of Immunopharmacology
- Vol. 2 (4) , 435-452
- https://doi.org/10.3109/08923978009026405
Abstract
Beta-adrenergic binding sites have been identified and characterized on murine lymphocytes. The binding sites are of two distinct classes, a high affinity class which has an apparent dissociation constant of about 1 × 10−9M and numbers about 500 sites per cell, and a higher capacity binding class that appears to be more characteristic of a non-specific binding site. The binding of a beta-adrenergic radioligand at concentrations about equal to the Kd (dissociation constant) for the high affinity site can be competed for with classical adrenergic ligands, is reversible and does not display cooperative interactions.Keywords
This publication has 9 references indexed in Scilit:
- Cell cycle-specific changes in β-adrenergic receptor concentrations in C6 glioma cellsBiochemical and Biophysical Research Communications, 1980
- [3H]Dihydroalprenolol binding sites in rat myocardium: Relationship between a single binding site population and the concentration of radioligandBiochemical and Biophysical Research Communications, 1979
- Evidence for negative cooperativity among β-adrenergic receptors in cardiac and lung tissue of guinea pigBiochemical Pharmacology, 1979
- Reduced number of β-adrenergic receptors in the myocardium of spontaneously hypertensive ratsBiochemical and Biophysical Research Communications, 1978
- Chronic guanethidine treatment increases cardiac β-adrenergic receptorsNature, 1978
- Elevated beta-adrenergic receptor number after chronic propranolol treatmentBiochemical and Biophysical Research Communications, 1977
- β-Adrenergic receptors: Evidence for negative cooperativityBiochemical and Biophysical Research Communications, 1975
- The Binding Characteristics and Number of β-Adrenergic Receptors on the Turkey ErythrocyteProceedings of the National Academy of Sciences, 1974
- Pharmacologic Regulation of Antibody Release in Vitro: Effects of Vasoactive Amines and Cyclic AMPPublished by Springer Nature ,1974