Tetraplex Formation of a Guanine-Containing Nonameric DNA Fragment
- 26 October 1990
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 250 (4980) , 543-546
- https://doi.org/10.1126/science.2237404
Abstract
A combination of spectroscopic and calorimetric techniques has been used to characterize the structures formed by a family of short, guanine-containing DNA single strands of the form d[GGTTXTTGG], X = A, C, G, T. In 1 molar NaCl at low temperatures, these molecules do not behave like single strands, but rather exhibit properties consistent with tetraplex formation. The standard state enthalpies, entropies, and free energies for formation of each tetraplex have been measured, as have preliminary nuclear magnetic resonance (NMR) spectra. In 1 molar KCl, the melting behavior of the structure or structures is more complex than in 1 molar NaCl. This observation may be related to the recently proposed "sodium-potassium switch."This publication has 31 references indexed in Scilit:
- A sodium-potassium switch in the formation of four-stranded G4-DNANature, 1990
- A study of the structure of the lyomesophases formed by the dinucleoside phosphate d(GpG). An approach by x-ray diffraction and optical microscopyJournal of the American Chemical Society, 1989
- Thermodynamic comparison of the base pairs formed by the carcinogenic lesion O6-methylguanine with reference both to Watson-Crick pairs and to mismatched pairsBiochemistry, 1989
- An unusual DMA structure detected in a telomeric sequence under superhelical stress and at low pHNature, 1989
- A G4-DNA/B-DNA junction at codon 12 of c-Ha-ras is actively and asymmetrically methylated by DNA (cytosine-5)methyltransferaseBiochemical and Biophysical Research Communications, 1989
- Human telomeres: fusion and interstitial sitesTrends in Genetics, 1989
- Formation of parallel four-stranded complexes by guanine-rich motifs in DNA and its implications for meiosisNature, 1988
- Poly(inosinic acid) helixes: essential chelation of alkali metal ions in the axial channelBiochemistry, 1982
- X-ray study by fiber diffraction methods of a self-aggregate of guanosine-5′-phosphate with the same helical parameters as poly(rG)Journal of Molecular Biology, 1976
- X-ray fiber diffraction and model-building study of polyguanylic acid and polyinosinic acidJournal of Molecular Biology, 1975