Density‐dependent induction of TNF‐α release from human monocytes by immobilized P‐selectin

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Abstract
P‐selectin purified from human platelets, when immobilized on a solid surface, induced monocytes to release tumor necrosis factor‐α (TNF‐α). The induction of TNF‐α release was dependent on the concentration of P‐selectin used for the immobilization, and the maximal stimulation was observed when the plate was coated with 0.3 μg/ml of P‐selectin. Use of either a higher or a lower concentration of P‐selectin for the plate‐coating was found to elicit less TNF‐α release, although the higher concentration of P‐selectin caused a stronger adhesion of HL‐60 leukemic cells. The expression of mRNA for TNF‐α roughly paralleled the TNF‐α secretion, as assessed by RT‐PCR. These results indicate that monocytes are activated by immobilized P‐selectin in a density‐dependent manner.

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