A rapid quantitated viability test for transplant kidneys – Ready for human trial

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Abstract
A simple tetrazolium reduction test employing a small kidney biopsy is described by which ischemic damage produced by 10‐ to 30‐minute room temperature ischemia was readily detected. Four photometric measurements were taken 10 seconds apart and the linear reduction of the dye was plotted. The slope of the line produced after room temperature ischemia was significantly less than kidney biopsies taken in successive 10‐min intervals up to 1 hour (p < 0.001). The experiments were done in 5 mouse and 6 dog kidneys. Ischemia at 0, 10, 20, 30 and 37°C were also tested at 10, 20, 30, 60 min. Statistically significant differences were also noted at each of the temperature intervals. The tetrazolium test is therefore considered to be sensitive enough to measure damage to human kidneys which currently are transplanted with various cold and warm ischemia times.