Chromosome Studies on Polyploid Cell Strains ofChinese HamsterV: Banding Pattern

Open Access
Publisher Website
Google Scholar
Abstract
The authors have examined the « banding pattern », revealed by the Seabright method (1972), of chromosomes of an aneuploid-diploid line (C-125) and of certain lines of various degrees of ploidy derived from it. It was found that the line C-125 has preserved a large proportion of normal chromosomes, similar to those of the species, some in a diploid and others in a haploid condition. Analysis of the degree of homology of the chromosomes confirmed that, in the derived polyploid lines, part of the genome is in a triploid condition (Oliveri et al. 1972) even if in some cases only parts of the chromosome are in a triploid condition as a result of deletions. This last type of chromosome rearrangement is the one that has had the greatest importance in the evolution of the karyogram of the induced polyploid lines. The results are discussed in relation to problems of « dosage compensation ».