Effects of Annular Pupils on Confocal Fluorescent Imaging
- 1 September 1992
- journal article
- research article
- Published by Taylor & Francis in Journal of Modern Optics
- Vol. 39 (9) , 1883-1896
- https://doi.org/10.1080/09500349214551971
Abstract
For a confocal fluorescent microscope with annular lenses, the effects of varying the radii of central obstruction of the annular lenses on three, two and one-dimensional imaging have been investigated in terms of the optical transfer function. In particular, we consider the effects of the central obstruction on the signal strength for a system with either a point or finite-sized detector. The signal level as a function of the detector and the central obstruction sizes is presented for volume, planar and point objects, respectively.Keywords
This publication has 10 references indexed in Scilit:
- The significance of 3‐D transfer functions in confocal scanning microscopyJournal of Microscopy, 1992
- Confocal fluorescent microscopy with a finite-sized circular detectorJournal of the Optical Society of America A, 1992
- Three-dimensional Imaging in Confocal Fluorescent Microscopy with Annular LensesJournal of Modern Optics, 1991
- Improvement of axial resolution in confocal microscopy using an annular pupilOptics Communications, 1991
- Three-dimensional optical transfer function for an annular lensOptics Communications, 1991
- Signal strength and noise in confocal microscopy: Factors influencing selection of an optimum detector apertureScanning, 1991
- Three-dimensional optical-transfer-function analysis for a laser-scan fluorescence microscope with an extended detectorJournal of the Optical Society of America A, 1991
- Calculation of three-dimensional optical transfer function for a confocal scanning fluorescent microscopeJournal of the Optical Society of America A, 1989
- Optical sectioning in confocal fluorescent microscopesJournal of Microscopy, 1989
- Transfer Function for an Annular Aperture*Journal of the Optical Society of America, 1956