Studies on bacterial cell wall inhibitors. X. Properties of phospho-N-acetylmuramoyl-pentapeptide-transferase in peptidoglycan synthesis of Bacillus megaterium and its inhibition by amphomycin.
- 1 January 1982
- journal article
- research article
- Published by Japan Antibiotics Research Association in The Journal of Antibiotics
- Vol. 35 (9) , 1216-1221
- https://doi.org/10.7164/antibiotics.35.1216
Abstract
The phospho-N-acetylmuramoyl-pentapeptide-transferase from B. megaterium KM was characterized by the transfer reaction. The particulate enzyme preparation had the activity to transfer phospho-N-acetylmuramoyl-pentapeptide from UDP-N-acetylmuramoylpentapeptide to undecaprenoid-1-ol-phosphate. The optimum pH for activity was about 8.5. The reaction required the presence of Mg2+ and an SH-protector. With 25 mM Mg2+ the maximum activity was observed. The reaction was reversible and the addition of UMP decreased the formation of undecaprenoid-1-diphospho-N-acetylmuramoyl-pentapeptide. Amphomycin inhibited non-competitively the transferase for the substrate UDP-N-acetylmuramoyl-pentapeptide.This publication has 12 references indexed in Scilit:
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