Inhibition of 2‐Amino‐3, 8‐dimethylimidazo[4,5‐f]quinoxaline‐mediated DNA‐adduct Formation by Chlorophyllin in Drosophila
Open Access
- 1 April 1996
- journal article
- Published by Wiley in Japanese Journal of Cancer Research
- Vol. 87 (4) , 325-328
- https://doi.org/10.1111/j.1349-7006.1996.tb00224.x
Abstract
The effect of chlorophyllin on 2‐ammo‐3,8‐dimethylimidazo[4,5‐f]quinoxaline (MeIQx)‐mediated DNA‐adduct formation in Drosophila was studied. Third‐instar larvae of Drosophila were fed MeIQx at 1 mg/6.5 g‐feed/bottle, with or without chlorophyllin (100–300 ing). After a 6 h feeding exposure to MeIQx, the larvae were divided into 2 groups. The first group was examined for covalent DNA adducts by 32P‐postlabeIing assay. The second group was assayed for DNA damage by allowing the larvae to develop to adults and measuring the male/female ratio (males, DNA repair‐deficient; females, DNA repair‐proficient). The 32P‐postlabeling results indicated a significant decrease in DNA adduct levels in larvae treated with MeIQx and 300 mg chlorophyllin (1.7±0.7 adducts/107 nucleotides) as compared with MeIQx‐treated larvae (6.5±2.1 adducts/107 nucleotides). The results on male/female sex ratios also indicated a chlorophyllin‐indnced decrease in DNA damage by exposure to MeIQx. The suppressive effect of chlorophyllin on the genotoxic actions of a polycyclic mutagen, MeIQx, may be a result of complex formation between chlorophyllin and the mutagen.Keywords
This publication has 19 references indexed in Scilit:
- Inhibitory effect of chlorophyllin on PhIP-induced mammary carcinogenesis in female F344 ratsCarcinogenesis: Integrative Cancer Research, 1995
- Inhibitory activity of chlorophyllin on the genotoxicity of carcinogens in DrosophilaCancer Letters, 1994
- Detection of Guanine-C8-2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine Adduct as a Single Spot on Thin-layer Chromatography by Modification of the32P-Postlabeling MethodJapanese Journal of Cancer Research, 1994
- Binding of polycyclic planar mutagens to chlorophyllin resulting in inhibition of the mutagenic activityMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1993
- Inhibition of 2-amino-3-methylimidazo[4.5-f] (IQ)-DNA binding by chlorophyllin: studies of enzyme inhibition and molecular complex formationCarcinogenesis: Integrative Cancer Research, 1992
- Protection by chlorophyllin against the covalent binding of 2-amino-3-methylimidazo[4,5-f]qiiinoline (IQ) to rat liver DNACarcinogenesis: Integrative Cancer Research, 1992
- Chemopreventive properties of chlorophyllin: inhibition of aflatoxin B1 (AFB1)-DNA binding in vivo and anti-mutagenic activity against AFB1 and two heterocyclic amines in the salmonella mutagenicity assayCarcinogenesis: Integrative Cancer Research, 1991
- Dietary inhibitors of mutagenesis and carcinogenesisMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1988
- Inhibition of the mutagenicity of amino acid pyrolysis products by hemin and other biological pyrrole pigmentsBiochemical and Biophysical Research Communications, 1980
- Sensitivity of drosphila mutants to chemical carcinogensMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1979