Regulation of Organ Formation by Cytokinin and Auxin in Lilium Bulbscales Grown In Vitro

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Abstract
Regulation of organ formation by cytokinin and auxin was investigated in vitro using Lilium auratum Lindl. (wild species habituated in Japan) and Lilium speciosum Thunb. cv. “Uchida”. The interaction of α-naphthylacetic acid (NAA) and kinetin on bulbscale or root differentiation was examined. NAA and kinetin showed mainly individual but also some synergistic effects. The effects of benzyladenine (BA) and kinetin were compared and the result indicated that BA has a stronger physiological effect on organ formation than kinetin and that their effects on Lilium auratum and Lilium speciosum were BA or kinetin-specific. The action of kinetin on Lilium was affected by sucrose concentration and the strength of the Murashige and Skoog medium (MS medium), and thus their high concentrations inhibited the kinetin-induced bulbscale differentiation. Furthermore, a high sucrose level negated the kinetin inhibition of root formation, while high MS medium strength in itself inhibited root formation. Morphological observation of bulbscale differentiation induced under a high kinetin level revealed that the new-formed structures are homologous to normally grown bulbs in soil in spite of their particular features.

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