MULTI-COLOUR FLUORESCENCE METHOD IN CYTOFLUOROMETRY

1 January 1979

journal article
Published by Japan Society of Histochemistry & Cytochemistry in ACTA HISTOCHEMICA ET CYTOCHEMICA

Vol. 12 (3) , 257-272
https://doi.org/10.1267/ahc.12.257

Abstract

Due to the development of the post-irradiation method and understanding of the basic principles for fluorescence stablization, the most serious problems of primary fluorescence and fluorescence fading in cytofluorometry have been solved. These technical improvements enabled us to develop a multi-fluorescence method for quantification of different cellular constituents and metabolic products in the same cell. Besides the applications of the multifluorescence method described in the present paper, many other techniques for multiparameter analysis systems will be developed in the future. Thus fluorescence cytofluorometry becomes a promising technique for quantitative analysis in biology and medicince.

Keywords

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