Effet du phosphate et du calcium sur la croissance et l'accumulation ionique chez les champignons ectomycorhiziens en culture in vitro

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Abstract
The Pi and Ca influence on growth and ionic accumulation was studied in several ectomycorrhizal fungi (Pisolithus tinctorius included) grown in vitro. The optimal growth was obtained with H2PO4- concentrations much lower than those of usual media: it may be represented by an exponential function, the parameters (maximal growth and concentration of half-maximal growth) of which were calculated. For low phosphate and Ca concentrations in the medium the dry matter mass of the mycelium and their content of the 2 elements increased simultaneously. For high concentrations, an accumulation independent of growth was observed in the thalli. When the P. tinctorius mycelium accumulated phosphate and Ca in relatively large amounts, these accumulations seemed to be not correlated. Rather, the correlation between the accumulation of total P and that of K was significant. The ratio of acid-soluble P to total P decreased in the mycelium when the Pi concentration increased in the medium.

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