Light Microscopic Autoradiography Followed by Electron Microscopy

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Abstract
We have developed a technique whereby sections processed for autoradiography to localize a labelled cell can then be re-sectioned for examination of the same cell under the electron microscope (Fig. 1). The benefits of this method are 1) positive ultrastructural identification of the tagged cell, as the cell is visible throughout the re-sectioning procedure, 2) no unnecessary sectioning, as each block is known to contain tagged cells, and 3) rapid tissue processing and examination.

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