Abstract
Cloning trypanosomes clearly showed that at least two enzymically distinct populations of Trypanozoon were present within the salivary glands of a wild-caught Glossina palpalis palpalis from Ivory Coast. Normal sampling techniques detected only the predominant population, which would be the only population reaching a stage suitable for enzyme characterization. Thus a tsetse fly may fail to be implicated as a transmitter of man-infective trypanosomes. The enzymic relationship of the two Trypanozoon populations suggested genetic hybridization. The separate clones retained their individual enzymic integrity after blood and cyclic transmission.