Poly(A)-Containing RNA Molecules in Electrophoretically Separated Fractions of Rapidly Labeled Nuclear RNA from Unstimulated and PHA-Stimulated Human Lymphocytes

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Abstract
Agarose-polyacrylamide gel electrophoresis allows detailed resolution of the high molecular weight RNA labeled in small human lymphocytes after several hours of incubation in vitro with a radioactive precursor. Several discrete peaks, corresponding to S values of 32, 35–36, 40–42, 45, 50, 54–55, 58–60, 70–75, and 90 were consistently observed. In PHA-stimulated lymphocytes, no discrete fraction slower than the 45S RNA was identified when the cells were incubated for periods longer than 15 min. However, the relative occurrence of molecules containing poly(A) in fractions slower than 45S RNA was around 20% in both unstimulated and stimulated cells. Gel electrophoresis of poly(A)-rich RNA from unstimulated lymphocytes showed a heterogeneous distribution.

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