Estimation of lymphocyte percentage and number on the Coulter Counter, Model S Plus Phase II.

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Abstract
The Coulter Counter Model S Plus Phase II provided precise measurements of human lymphocyte percentage and count and carry-over was negligible. Lymphocyte percentage values agreed well with those from the stained blood film except when the percentage was high and in these circumstances the instrument gave underestimates. When making artificial mixtures with increasing lymphocyte count a progressive underestimation of the lymphocyte percentage was also noted. The display of leukocyte volume distribution was a useful attribute particularly when the instrument alerted the operator to rejected profiles. Rejection by the instrument was a helpful function occurring in the myeloid leukemias, erythroblastosis, and in some cases when the lymphocytes differed from normal, for example, glandular fever [mononucleosis] and chronic lymphocytic leukemia.