Skeletal Muscle Fiber Size and Capillarity

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Abstract
The ATPase technique following preincubation in an acid medium (pH 3.8-4.0) was used to visualize capillaries in guinea pigs, rabbits, cats and dogs. After preincubation at a pH of 4.5, in combination with the DPNH [NADH] tetrazolium reductase, it was used to identify fiber types. A positive correlation was demonstrated between fiber cross sectional area and C:F [capillary to fiber ratio], indicating that large fibers are surrounded by more capillaries. The increase in fiber size may or may not be associated with changes in capillary density.

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