Invasion of Malignant Cells into 51Cr-Labeled Host Tissues in Organotypical Culture

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Abstract
We have attempted to quantitate malignant invasion in vitro. Therefore we adapted the 51Cr-release cytotoxicity test to organotypical culture systems, that proved useful for morphological analysis of invasion. We counted 51Cr-release from labeled fragments of embryonic chick heart and mesonephros, cultured either on semisolid or in fluid medium, during their invasion by HeLa cells. Although demonstrated morphologically, malignant invasion did not bring about significant changes of 51Cr-release as compared to control cultures without HeLa cells. The failure of the 51Cr-release test in our experimental device might shed some light on the mechanisms of malignant invasion.

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