Cytosine Β-D Arabino-Furanoside (Ara-C) and CPT-11 inhibit cell proliferation in human colorectal cancer with microsatellite instability
- 15 July 2004
- journal article
- abstracts
- Published by American Society of Clinical Oncology (ASCO) in Journal of Clinical Oncology
- Vol. 22 (14_suppl) , 2061
- https://doi.org/10.1200/jco.2004.22.14_suppl.2061
Abstract
2061 Background: Recent studies suggest 5-Fluorouracil-based adjuvant chemotherapy does not improve survival in colorectal cancer (CRC) patients with microsatellite instability (MSI) (NEJM, 349 July: 247–257, 2003). We have reported decreased expression of p12DOC-1 is associated with increased proliferation and apoptosis arrest in human CRC with MSI (Oncogene, 22: 6304–10, 2003). The purpose of this study was to measure differential expression of p12DOC-1 using a Cancer Cell Line Profiling (CCLP) Array (Clontech) and to assess the efficacy of CPT-11 and oxaliplatin, in human MSI CRC cell lines. Methods: A CCLP array containing 702 complex cDNA samples from 26 different human cancer cell lines was treated with a range of chemotherapeutic agents. The array was hybridized with a p32-dCTP labeled p12DOC-1 probe. Array results were confirmed using real-time RT-PCR. In addition, the toxicity of CPT-11 and oxaliplatin (neither agent tested on array) against CRC cell lines, RKO (MSI) and SW480 (microsatellite sta...Keywords
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