Ion Selective Electrodes for Phosphate Using Enzyme Systems
- 1 March 1976
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 9 (3) , 277-289
- https://doi.org/10.1080/00032717608059102
Abstract
Two different enzyme electrodes for the specific assay of phosphate ion are described. One system utilizes the enzymes phosphorylase-a, phosphoglucomutase and glucose-6-phosphate dehydrogenase, the second glyceraldehyde-phosphate dehydrogenase, phosphoglycerate kinase and hexokinase. In both cases the assay is effected by amperometric monitoring of the NADH or NADPH formed in the reaction at an applied potential of +0. 65 V.Keywords
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