Recognition of Bergmann glial and ependymal cells in the mouse nervous system by monoclonal antibody.

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Abstract
A monoclonal antibody designated anti-Cl was obtained from a hybridoma clone isolated from a fusion of mouse myeloma NS1 with spleen cells from BALB/c mice injected with homogenate of white matter from bovine corpus callosum. In the adult mouse neuroectoderm, C1 antigen is detectable by indirect immunohistology in the processes of Bergmann glial cells (also called Golgi epithelial cells) in the cerebellum and of Muller cells in the retina, whereas other astrocytes that express glial fibrillary acidic protein in these brain areas are negative for C1. C1 antigen is expressed in most, if not all, ependymal cells and in larger blood vessels, but not capillaries. In the developing, early postnatal cerebellum, C1 antigen is not confined to Bergmann glial and ependymal cells but is additionally present in astrocytes of presumptive white matter and Purkinje cell layer. In the embryonic neuroectoderm, C1 antigen is expressed at day 10, the earliest stage tested. The antigen is distinguished in radially oriented structures in telencephalon, pons, pituitary anlage and retina. Ventricular cells are not labeled by C1 antibody at this stage. C1 antigen is not detectable in astrocytes of adult or nearly adult cerebella from the neurological mutant mice staggerer, reeler and weaver, but is present in ependymal cells and larger blood vessels. C1 antigen is expressed not only in the intact animal but also in cultured cerebellar astrocytes and fibroblastlike cells. It is localized intracellularly.