ALDEHYDE FORMATION IN THE LIPIDE DROPLETS OF THE ADRENAL CORTEX DURING FIXATION, AS DEMONSTRATED CHEMICALLY AND HISTOCHEMICALLY

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Abstract
Histochemically demonstrable carbonyl groups in lipid droplets of cells of the adrenal cortex are concluded to be aldehydes produced by autoxidation of unsaturated fatty acids. The evidence includes the inhibition or depression of carbonyl reaction by antioxidants and cholating agents, the consistency of hydrazide reactivity only with reactivity to Schiff reagent, and the appearance of carbonyl groups with the disappearance of lipid unsaturation. Ketonic lipids present in sections treated with halogens appear to be oxidative artifacts, following hypophysectomy there is a decline in unsaturated lipids in adrenal cortex which probably explains past results with carbonyl reaction. Fixatives containing antioxidants are recommended.