Phosphate-Regulated Inactivation of the Kinase PHO80-PHO85 by the CDK Inhibitor PHO81
- 7 October 1994
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 266 (5182) , 122-126
- https://doi.org/10.1126/science.7939631
Abstract
A complex consisting of the cyclin-dependent kinase (CDK) PHO85 and the cyclin PHO80 phosphorylates and is thought to inactivate the transcription factor PHO4 when yeast cells are grown in medium containing high concentrations of phosphate. The CDK inhibitor PHO81 inhibits the kinase activity of the PHO80-PHO85 complex when Saccharomyces cerevisiae cells are grown in medium depleted of phosphate. A region of PHO81 with similarity to the mammalian CDK inhibitor p16INK4 is sufficient for inhibition in vitro. These studies demonstrate that CDK inhibitors are used to regulate kinases involved in processes other than cell cycle control and suggest that the ankyrin repeat motif may be commonly used for interaction with cyclin-CDK complexes.Keywords
This publication has 32 references indexed in Scilit:
- Direct Inhibition of the Yeast Cyclin-Dependent Kinase Cdc28-Cln by Far1Science, 1994
- Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signalsCell, 1994
- A Cell Cycle Regulator Potentially Involved in Genesis of Many Tumor TypesScience, 1994
- Phosphorylation of the Transcription Factor PHO4 by a Cyclin-CDK Complex, PHO80-PHO85Science, 1994
- p21 is a universal inhibitor of cyclin kinasesNature, 1993
- A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4Nature, 1993
- The ANK repeat: a ubiquitous motif involved in macromolecular recognitionTrends in Cell Biology, 1992
- Identification of a gene necessary for cell cycle arrest by a negative growth factor of yeast: FAR1 is an inhibitor of a G1 cyclin, CLN2Cell, 1990
- Analysis of cDNA for human erythrocyte ankyrin indicates a repeated structure with homology to tissue-differentiation and cell-cycle control proteinsNature, 1990
- The structure of an antigenic determinant in a proteinCell, 1984