Regional cerebral blood flow in rats with cerebral ischemia produced by bilateral vertebral and carotid artery occlusion.

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Abstract
Regional cerebral blood flow (rCBF) was measured prior to, during and after global cerebral ischemia in rats. Global cerebral ischemia was produced by 10 or 30-min occlusion of both common carotid arteries which was done 24 h after the permanent electrocauterization of bilateral vertebral arteries. The rCBF was measured using the radioactive microsphere technique. In rats subjected to 10-min cerebral ischemia, rCBF in 9 brain regions was reduced to 11.3-54.8% (mean: 26.9%) of that of the sham operated control. Ten minutes after recirculation, hyperperfusion was observed in the cerebral cortex, hippocampus and striatum. A moderate recovery was detected in the accumbens nucleus + olfactory tuberculum, thalamus and hypothalamus. However, rCBF in these 6 regions was again decreased 20-30 min later, and it recovered to levels > 50% of the control 60 min after the ischemic event. In the other 3 regions (cerebellum, superior and inferior colliculus, pons + medulla), rCBF increased toward the control level gradually, and it completely recovered 60 min after recirculation. In rats subjected to 30 min cerebral ischemia, rCBF in 9 brain regions was reduced to 1.77-26.3% (mean: 9.6%) of that of the control. The post-ischemic hyperperfusion in the cerebral cortex and hippocampus and a moderate recovery of rCBF in the striatum and accumbens nucleus + olfactory tuberculum were observed 10 min after the cerebral ischemia. rCBF in these 4 regions remained under the control levels from 20-60 min after recirculation. The change of rCBF in the other regions was similar to that in the 10-min ischemic group, but the recovery was less at 60 min after recirculation. The abnormalities in the post-ischemic rCBF may be involved in the behavioral, EEG and neurochemical changes observed after the cerebral ischemia.