Identification of Products and Intermediates During L-Dopa Oxidation to Dopachrome Using High Pressure Liquid Chromatography with Electrochemical Detection
- 1 April 1993
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 16 (5) , 1117-1133
- https://doi.org/10.1080/10826079308019575
Abstract
Products and intermediates produced during L-dopa oxidation by sodium periodate and mushroom tyrosinase were studied using high pressure liquid chromatography with electrochemical detection (HPLC-ED). The oxidation products and intermediates, including dopaquinone, leukodopachrome, 2, 4, 5-trihydroxyphenylalanine and its quinone species, were readily separated on a reverse-phase column with 15 mM citrate buffer, pH 3.0, containing 35 mM NaCl, 0.25 mM octyl sulfate and 0.7 mM Na2EDTA and detected at either a reductive (-100 mV) or an oxidative potential (750 mV) of the working electrode. Using these techniques, the effect of base and acid on the chemical reaction of dopaquinone to dopachrome was demonstrated.Keywords
This publication has 2 references indexed in Scilit:
- Kinetic study on the effect of pH on the melanin biosynthesis pathwayBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1991
- A kinetic study of the melanization pathway between L-tyrosine and dopachromeBiochimica et Biophysica Acta (BBA) - General Subjects, 1987