Studies on the biological role of DNA methylation. II. Role of 174 DNA methylation in the process of viral progeny DNA synthesis
- 1 October 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 3 (10) , 2665-2676
- https://doi.org/10.1093/nar/3.10.2665
Abstract
In vivo inhibition of bacteriophage Φχ174 DNA methylation by nicotinamide resulted in the accumulation of replicative intermediates with multiple-genome length single-stranded “tails”. These abnormal replicative intermediates could not be chased into viral single-stranded circular DNA. The effect of nicotinamide on phage maturation and accumulation of abnormal replicative intermediates could be reversed by washing out the inhibitor. The results suggest that the single methyl group present in the viral DNA serves as a recognition site for a specific endonuclease, probably the gene A protein product, that is responsible for the excision of the single-stranded one-genome long viral DNA, before final maturation of the virus occurs.Keywords
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