Photolabile Antibiotics as Probes of Ribosomal Structure and Function *
- 1 June 1980
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 346 (1) , 302-323
- https://doi.org/10.1111/j.1749-6632.1980.tb22105.x
Abstract
In summary, our studies have resulted in the development of new reagents and synthetic procedures for the introduction of both radioactivity and photolability into antibiotics; have provided examples of how PAGE analysis can be used to conveniently test various aspects of photoaffinity labeling of complex receptors, such as the ribosome; have revealed examples of unexpected photoreactivity, as in the case of puromycin; and have provided strong evidence regarding the three-dimensional location of the peptidyl transferase center and the site of that interaction of the 3' and of aminoacyl-tRNA with the 30S subunit. Work that is now in progress should provide similar detailed information regarding other functional sites in the ribosome.Keywords
This publication has 46 references indexed in Scilit:
- Identification of fifteen neighboring protein pairs in the Escherichia coli 50 S ribosomal subunit crosslinked with 2-iminothiolaneJournal of Molecular Biology, 1979
- Ribosomal proteins localized at a single region of the large subunit by immune electron microscopyJournal of Molecular Biology, 1978
- Photoaffinity labeling ofEscherichia coliribosomes with an aryl azide analogue of puromycinFEBS Letters, 1978
- Ribosomal proteins S5, S11, S13 and S19 localized by ekectron microscopy of antibody-labeled subunitsJournal of Molecular Biology, 1975
- p‐nitrophenylalanine, p‐azidophenylalanine, m‐azidophenylalanine, and o‐nitro‐p‐azido‐phenylalanine as photoaffinity labelsFEBS Letters, 1974
- Inhibitors of protein synthesisFEBS Letters, 1974
- Protein involved in the binding of dihydrostreptomycin to ribosomes of Escherichia coliJournal of Molecular Biology, 1973
- Reaction of N-ethyl maleimide with the ribosomes of Escherichia coliJournal of Molecular Biology, 1971
- Binding of dihydrostreptomycin to ribosomal subunitsJournal of Molecular Biology, 1968
- Binding of tetracycline to the 30S ribosomes and to polyuridylic acidBiochemical and Biophysical Research Communications, 1965