Iodine-131 Labeling of Purified Microbial Antigens by Microdiffusion
- 1 October 1967
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 126 (1) , 282-284
- https://doi.org/10.3181/00379727-126-32425
Abstract
Conventional methods for introduction of I-133 may modify or destroy the serological reactivity of labile microbial antigens. A modified microdiffusion iodination method that eliminates the exposure of antigens to excess oxidizing agent produced satisfactory trace labeling of a purified protective angiten of Bacillus anthracis and enterotoxin-B of Staphylococcus aureus with-out detectable change in precipitating activity. The iodinated antigens are suitable for use in the (NH4)2SO4 coprecipitation technique of Farr.This publication has 0 references indexed in Scilit: